The purpose of this study is to develop a new method which enables the goat ESR to be used as an effective clinical test. Blood samples were taken from 61 Korean native goats aged above one year old and the effect of tube inclinations, tube bores, tube lengths, environmental temperatures during tests and packed erythrocyte volumes (PCV) on the ESR were observed. The results were summarized as follows.
1. The ESR/hr using capillary hematocrit tube (Micro-Ht-tube) was gradually increased as the tube angle inclined from 90 (vertical) to 15 degrees and the best angle in view of both security and fast sedimentation rate was found to be an angle of 45 degrees.
2. The 45-degree angled ESR (45°-ESR) increased as the diameter of tube bore decreased.
3. The tube length did not affect the 45°-ESR in percent.
4. The 45°-ESR increased with the increased environmental temperature during the ESR test.
5. The heparinized Micro-Ht tubes did not affect the 45°-ESR of EDTA-blood in healthy group but in anemic group. In the anemic group, the ESRs by the heparinized Micro-Ht-tubes were slightly higher than those by non-heparinized Micro-Ht-tubes.
6. By using the autologous plasma, PCV of the blood was adjusted to be 10, 20, 30, 40 and 50㎖/100㎖ and 45°-ESRs were determined in the Micro-Ht-tubes. The 45°-ESRs increased as the values of PCV decreased. The regression of the 45°ESR to PCV was curvilinear with the second degree polynomial, Y=42.1838-1.7355X+0.0180X^2 (r=0.9997).
7. The 45°-ESR/hr, using non-heparinized Micro-Ht-tubes at 20℃, was determined in 35 healthy Korean native goats. The average PCV was 30.6±1.4㎖/100㎖. The observed ESR values averaged 6.8±1.7% and the corrected ESR values to the standard PCV of 31㎖/100㎖ averaged 6.5±1.2%.
From these results, the angled capillary tube method was found to be desirable ESR test of goat blood in which EDTA-blood is filled in nonheparinized Micro-Ht-tubes held at an angle of 45 degrees for an hour at 20℃.
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